Development of A Rapid Method for the Diagnosis of Citrus Greening Disease using the Polymerase Chain Reaction
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چکیده
The fastidious bacterium causing citrus greening disease occurs in uneven and low concentrations in the sieve tubes of host plants[ A rapid and sensitive assay based on the polymerase chain reaction "PCR# has been developed using the primers derived from the sequences of the cloned DNA fragment of greening fastidious bacterium "GFB# to detect GFB infection in citrus[ One set of the primer pairs "named 115!primer pair#\ which generates a 115 bp GFB!speci_c fragment from total DNA templates puri_ed from diseased citrus plants\ was tested and chosen for PCR ampli_cation[ The PCR!based assay using this 115!primer pair e}ectively detected GFB infec! tion in various citrus cultivars collected from di}erent Asian countries[ This detection technique\ which can be completed within 5 h\ o}ers a rapid and e.cient method for accurate diagnosis of citrus greening disease[
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